Phase Separation Methods and Chromatography

University-of-Soukahras

This course introduces students to the fundamental techniques of phase separation and the essential principles of chromatographic analysis, which are widely used in analytical and industrial chemistry. Students will explore the theoretical background, practical procedures, and applications of these methods in separating, identifying, and quantifying components in complex mixtures. The course covers classical separation techniques such as extraction, distillation, filtration, crystallization, and centrifugation, which form the basis for effective sample preparation. It then transitions to a detailed study of chromatographic methods including Thin-Layer Chromatography (TLC), High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), and others.

By the end of the course, students will be able to: Understand the principles behind phase separation and chromatographic processes. Select and apply appropriate separation techniques based on sample type and analysis goals. Operate basic laboratory equipment and chromatographic instruments. Analyze and interpret chromatograms and separation results. Apply these methods to real-world samples in environmental, pharmaceutical, or food chemistry.

This course is designed for 3rd-year undergraduate students in analytical chemistry, environmental sciences, or any related field that requires strong competence in chemical analysis and separation techniques.

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